Complete Peptide Dosing Guide: How to Calculate and Administer

Reconstitution Basics

Most peptides arrive as lyophilized (freeze-dried) powder and must be reconstituted with bacteriostatic water before use.

Step-by-Step Reconstitution

Clean the vial tops with an alcohol swab

Draw the required amount of bacteriostatic water into your syringe

Inject slowly down the side of the peptide vial — never directly onto the powder

Swirl gently — never shake

Allow 5 minutes for full dissolution

Calculating Your Dose

Formula: (Desired dose in mcg ÷ Total peptide in vial in mcg) × Volume of water added in mL × 1000 = Units on insulin syringe

Example: 2mg vial + 2mL water. For a 200mcg dose: (200 ÷ 2000) × 2 × 1000 = 20 units

Syringe Selection

Use U-100 insulin syringes (29-31 gauge) for subcutaneous injections. These minimize discomfort and are the industry standard for peptide administration.

Injection Sites

Best subcutaneous sites: abdomen (2 inches from navel), outer thigh, or upper arm. Rotate sites to prevent lipodystrophy.

Storage After Reconstitution

Reconstituted peptides should be refrigerated (2-8°C) and used within 30 days. Never freeze reconstituted peptides.

Conclusion

Proper reconstitution and dosing is critical for both safety and efficacy. When in doubt, start with conservative doses and titrate upward.